Publications

Journal covers

Top 3 proudest papers! 

Nicolas, W.J., Fäßler, F., Dutka, P., Schur, F.K.M., Jensen, G., Meyerowitz, E., 2022. Cryo-electron tomography of the onion cell wall shows bimodally oriented cellulose fibers and reticulated homogalacturonan networks. Current Biology 32, 2375-2389.e6. Link to article 

Nicolas W, Grison MS, Trepout S, Gaston A, Fouché M, Cordelières FP, Oparka KJ, Tilsner J, Brocard L, Bayer EM. 2017. Architecture and permeability of post-cytokinesis plasmodesmata lacking cytoplasmic sleeves. Nature Plants 3. 17082 (2017). Link to article  

Kaplan, M., Chang, Y.-W., Oikonomou, C.M., Nicolas, W.J., Jewett, A.I., Kreida, S., Dutka, P., Rettberg, L.A., Maggi, S., Jensen, G.J., 2023. Dynamic structural adaptations enable the endobiotic predation of bdellovibrio bacteriovorus. Nat Microbiol 8, 1267–1279 (2023).  Link to article 

Cryo-ET papers

Nicolas, W.J., Fäßler, F., Dutka, P., Schur, F.K.M., Jensen, G., Meyerowitz, E., 2022. Cryo-electron tomography of the onion cell wall shows bimodally oriented cellulose fibers and reticulated homogalacturonan networks. Current Biology 32, 2375-2389.e6. Link to article 

Nicolas WJ, Jensen G.J., Meyerowitz E, 2022. Cryo-electron tomography to study the structure of the primary cell wall in Allum cepa. Bio-protocol. Link to paper 

William J. Nicolas, Debnath Ghosal, Elitza I. Tocheva, Elliot M. Meyerowitz, Grant J. Jensen. Structure of the Bacterial Cellulose Ribbon and Its Assembly-Guiding Cytoskeleton by Electron Cryotomography. Journal of Bacteriology Jan 2021, 203 (3). Link to paper 

Kaplan, M., Chang, Y.-W., Oikonomou, C.M., Nicolas, W.J., Jewett, A.I., Kreida, S., Dutka, P., Rettberg, L.A., Maggi, S., Jensen, G.J., 2023. Dynamic structural adaptations enable the endobiotic predation of bdellovibrio bacteriovorus. Nat Microbiol 8, 1267–1279 (2023).  Link to article 

Kaplan, M., Chreifi, G., Metskas, L.A., Liedtke, J., Wood, C.R., Oikonomou, C.M., Nicolas, W.J., Subramanian, P., Zacharoff, L.A., Wang, Y., Chang, Y.-W., Beeby, M., Dobro, M., Zhu, Y., McBride, M., Briegel, A., Shaffer, C., Jensen, G.J., 2021. In situ imaging of bacterial outer membrane projections and associated protein complexes using electron cryo-tomography. eLife 10. Link to paper 

Mohammed Kaplan, William J Nicolas, Wei Zhao, Stephen D carter, Lauren Ann Metskas, Georges Chreifi, Debnath Ghosal and Grant J Jensen. In Situ Imaging and Structure Determination of Biomolecular Complexes Using Electron Cryo-Tomography. In: Gonen T., Nannenga B.L. (eds) cryoEM. Methods in Molecular Biology, vol 2215. Humana, New York, NY. Link to paper 

MicroED papers

Gillman, C., Nicolas, W. J., Martynowycz, M. W. & Gonen, T. (2023). Design and implementation of suspended drop crystallization. IUCrJ, 10, 430-436.. Link to paper 

Martynowycz, M.W., Shiriaeva, A., Clabbers, M.T.B., Nicolas, W.J., Weaver, S.J., Hattne, J., Gonen, T., 2023. A robust approach for MicroED sample preparation of lipidic cubic phase embedded membrane protein crystals. Nature communications 14, 1086 (2023). Link to paper 

Anna Shiriaeva, Michael W. Martynowycz, William J. Nicolas, Vadim Cherezov, Tamir Gonen. MicroED structure of the human vasopressin 1B receptor. bioRxiv 2023.07.05.547888. Link to paper 

Plastic section electron tomography papers

Nicolas W, Grison MS, Trepout S, Gaston A, Fouché M, Cordelières FP, Oparka KJ, Tilsner J, Brocard L, Bayer EM. 2017. Architecture and permeability of post-cytokinesis plasmodesmata lacking cytoplasmic sleeves. Nature Plants 3. Link to paper 

Nicolas WJ, Bayer EM, Brocard L. 2017. The Study of Three-dimensional Structure of Plasmodesmata in Plant Tissues by Electron Tomography. From High Pressure Freezing Preparation to EM Ultrathin Section on-grid Deposition. Bio-protocol 7. Link to paper 

Nicolas W, Grison MS, Bayer EM. 2018. Shaping plasmodesmata intercellular channels: The structure-to-function missing link. Journal of Experimental Botany 69.  Link to paper 

Yan, D., Yadav, S. R., Paterlini, A., Nicolas, W. J., Belevich, I., Grison, M. S., … Bayer, E. M. (2019). PLM modulates phloem unloading through sphingolipid biosynthesis and plasmodesmal ultrastructure. Nature Plants, 5(6), 604–615. Link to paper 

Brault, M. L., Petit, J. D., Immel, F., Nicolas, W. J., Glavier, M., Brocard, L., … Bayer, E. M. (2019). Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata. EMBO Reports. Link to paper 

Petit, Jules D., Li, Ziqiang Patrick, Nicolas, William J., Grison, Magali S. & Bayer, Emmanuelle M. Dare to change, the dynamics behind plasmodesmata-mediated cell-to-cell communication. Current Opinion in Plant Biology vol. 53 80–89 (2020). Link to paper 

Tilsner J, Nicolas W, Rosado A, Bayer EM. 2016. Staying tight: plasmodesmal membrane contact sites and the control of cell-to-cell connectivity in plants. Annual Review of Plant Biology 67, 1–28. Link to paper 

Grison, M.S.a, Brocard, L.a, Fouillen, L., Nicolas, W., Wewer, V., Dörmann, P., Nacir, H., Benitez-Alfonso, Y., Claverol, S., Germain, V., et al. (2015). Specific membrane lipid composition is important for plasmodesmata function in Arabidopsis. The Plant Cell 27, 1228–1250;    aco-first authors Link to paper